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    歐盟評(píng)估轉(zhuǎn)基因大腸桿菌菌株EBASSC生產(chǎn)的交替糖蔗糖酶的安全性
    日期:2025-04-10  來(lái)源:食品伙伴網(wǎng)
       食品伙伴網(wǎng)訊  2025年4月9日,歐盟食品安全局就一種交替糖蔗糖酶(alternansucrase)的安全性評(píng)價(jià)發(fā)布意見(jiàn)。
     
      據(jù)了解,這種食品酶是由轉(zhuǎn)基因大腸桿菌菌株EBASSC生產(chǎn)的,旨在用于糖的加工,以生產(chǎn)麥芽糖交替糖寡糖。
     
      經(jīng)過(guò)評(píng)估,專(zhuān)家小組認(rèn)為,在預(yù)期的使用條件下,不能排除飲食暴露引起過(guò)敏反應(yīng)的風(fēng)險(xiǎn),但這種可能性被認(rèn)為很低。根據(jù)所提供的數(shù)據(jù),評(píng)估小組得出結(jié)論,這種食品酶在預(yù)期使用條件下不會(huì)引起安全問(wèn)題。部分原文報(bào)道如下:
     
      The food enzyme alternansucrase (sucrose:1,6(1,3)-α-d-glucan 6(3)-α-d-glucosyltransferase; EC 2.4.1.140) is produced with the genetically modified Escherichia coli strain EBASSC by Advanced Enzyme Technologies Ltd. The production strain of the food enzyme contains a known antimicrobial resistance gene. However, based on the absence of viable cells and DNA from the production organism in the food enzyme, this is not considered to be a risk. The food enzyme is intended to be used in the processing of sugars for the production of maltose alternan gluco-oligosaccharides. Two sets of dietary exposure estimates were made, using the default or more specific input factors. The refined dietary exposure was estimated to be up to 0.586 mg total organic solids (TOS)/kg body weight (bw) per day in European populations. Genotoxicity tests did not indicate a safety concern. The systemic toxicity was assessed by means of a repeated dose 90-day oral toxicity study in rats. The Panel identified a no observed adverse effect level of 500 mg TOS/kg bw per day, the mid-dose tested, which when compared with the refined exposure estimate, results in a margin of exposure of at least 853. A search for the homology of the amino acid sequence of the alternansucrase to known allergens was made and no match was found. The Panel considered that a risk of allergic reactions upon dietary exposure to the food enzyme cannot be excluded, but the likelihood is low. based on the data provided, the Panel concluded that this food enzyme does not give rise to safety concerns, under the intended conditions of use.
     
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